Publication
Title
The biocontrol agent Lactiplantibacillus plantarum AMBP214 is dispersible to plants via bumblebees
Author
Abstract
Microbial biocontrol agents have emerged as a promising alternative for the management of plant diseases and the reduction of chemical pesticide dependence. However, a significant challenge in using these agents is their inconsistent performance under field conditions, often caused by the poor establishment and limited spread of microorganisms. Entomovectoring, a system where microbial biocontrol agents are dispersed to crops via pollinators, provides a potential solution to these challenges. Still, there are limited examples of successful systems, and no studies have tested this technology with versatile and generally beneficial lactobacilli. Here, we demonstrate that Lactiplantibacillus plantarum AMBP214 shows potential as a biocontrol agent displaying antimicrobial activity in an in vitro and a gnotobiotic seedling experiment, and the ability to establish itself in strawberry flowers. An innovative formulation and dispersal strategy were proven successful with this strain, as it could be formulated into a spray-dried powder that could be loaded onto bumblebees via a dispenser. When loaded bumblebees were released into a greenhouse with strawberry plants, L. plantarum AMBP214 was effectively dispersed to flowers, resulting in high bacterial abundances (on average 1 x 10(5) CFUs per flower) and consistent coverage across all sampled flowers. We could not show a protective effect of AMBP214 against Botrytis cinerea in a greenhouse trial with strawberry flowers under the tested conditions, so that further screening of other strains against this major pathogen will be necessary. However, our study provides the necessary proof of concept data that spray-dried lactobacilli can be used with entomovectoring, providing a novel and promising approach to biocontrol. These results pave the way for further research and development of spray-dried non-spore-forming bacteria in entomovectoring strategies, which hold great promise for enhancing plant health and mitigating the negative impacts of plant diseases.
Language
English
Source (journal)
Applied and environmental microbiology. - Baltimore, Md
Publication
Washington : Amer soc microbiology , 2023
ISSN
0099-2240
DOI
10.1128/AEM.00950-23
Volume/pages
(2023) , p. 1-17
ISI
001099067600001
Pubmed ID
37882529
Full text (Publisher's DOI)
Full text (open access)
The author-created version that incorporates referee comments and is the accepted for publication version Available from 26.04.2024
UAntwerpen
Faculty/Department
Research group
Project info
Launch of a microbiome service platform.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 04.12.2023
Last edited 08.12.2023
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