Publication
Title
Biophysical characterization of the Plasmodium falciparum circumsporozoite protein's N‐terminal domain
Author
Abstract
The circumsporozoite protein (CSP) is the main surface antigen of the Plasmodium sporozoite (SPZ) and forms the basis of the currently only licensed anti‐malarial vaccine (RTS,S/AS01). CSP uniformly coats the SPZ and plays a pivotal role in its immunobiology, in both the insect and the vertebrate hosts. Although CSP's N‐terminal domain (CSP N ) has been reported to play an important role in multiple CSP functions, a thorough biophysical and structural characterization of CSP N is currently lacking. Here, we present an alternative method for the recombinant production and purification of CSP N from P. falciparum (PfCSP N ), which provides pure, high‐quality protein preparations with high yields. Through an interdisciplinary approach combining in‐solution experimental methods and in silico analyses, we provide strong evidence that PfCSP N is an intrinsically disordered region (IDR) displaying some degree of compaction.
Language
English
Source (journal)
Protein science. - Cambridge
Publication
Cambridge : 2024
ISSN
0961-8368
DOI
10.1002/PRO.4852
Volume/pages
31 :1 (2024) , p. 1-17
Article Reference
e4852
ISI
001129406200001
Pubmed ID
38059674
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Project info
Investigation of the structural and functional role of the Plasmodium falciparum circumsporozoite protein in the development of liver stage malaria.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 09.12.2023
Last edited 16.02.2024
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