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Title
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Biophysical characterization of the Plasmodium falciparum circumsporozoite protein's N‐terminal domain
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Author
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Abstract
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| The circumsporozoite protein (CSP) is the main surface antigen of the Plasmodium sporozoite (SPZ) and forms the basis of the currently only licensed anti‐malarial vaccine (RTS,S/AS01). CSP uniformly coats the SPZ and plays a pivotal role in its immunobiology, in both the insect and the vertebrate hosts. Although CSP's N‐terminal domain (CSP N ) has been reported to play an important role in multiple CSP functions, a thorough biophysical and structural characterization of CSP N is currently lacking. Here, we present an alternative method for the recombinant production and purification of CSP N from P. falciparum (PfCSP N ), which provides pure, high‐quality protein preparations with high yields. Through an interdisciplinary approach combining in‐solution experimental methods and in silico analyses, we provide strong evidence that PfCSP N is an intrinsically disordered region (IDR) displaying some degree of compaction. |
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Language
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English
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Source (journal)
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Protein science. - Cambridge
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Publication
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Cambridge
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2024
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ISSN
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0961-8368
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DOI
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10.1002/PRO.4852
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Volume/pages
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31
:1
(2024)
, p. 1-17
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Article Reference
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e4852
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ISI
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001129406200001
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Pubmed ID
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38059674
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Full text (Publisher's DOI)
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Full text (open access)
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