Publication
Title
Heterologous expression of the cryptic mdk gene cluster and structural revision of maduralactomycin A
Author
Abstract
After conducting an in silico analysis of the cryptic mdk cluster region and performing transcriptomic studies, an integrative Streptomyces BAC Vector containing the mdk gene sequence was constructed. The heterologous expression of the mdk cluster in Streptomyces albus J1074 resulted in the production of the angucyclic product, seongomycin, which allowed for the assesment of its antibacterial, antiproliferative, and antiviral activities. Heterologous production was further confirmed by targeted knock-out experiments involving key regulators of the biosynthetic pathways. We were further able to revise the core structure of maduralactomycin A, using a computational approach.
Language
English
Source (journal)
RSC advances
Publication
2023
ISSN
2046-2069
DOI
10.1039/D3RA05931F
Volume/pages
13 :48 (2023) , p. 34136-34144
ISI
001108202500001
Pubmed ID
38019997
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 11.12.2023
Last edited 16.02.2024
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