Publication
Title
Microscale Thermophoresis (MST) to Study Rapid Alkalinization Factor (RALF)-Receptor Interactions
Author
Abstract
Microscale thermophoresis (MST) is a simple but powerful tool to study the in vitro interaction among biomolecules, and to quantify binding affinities. MST curves describe the change in the fluorescence level of a fluorescent target as a result of an IR-laser-induced temperature change. The degree and nature of the change in fluorescence signal depends on the size, charge, and solvation shell of the molecules, properties that change in function of the binding of a ligand to the fluorescent target. We used MST to describe the interaction between components of a regulatory module involved in plant cell wall integrity control. This module comprises the secreted peptide Rapid Alkalinization Factor 23 (RALF23) and its receptor complex consisting of the GPI-anchored receptor Lorelei-Like Glycoprotein 1 (LLG1) and a receptor kinase of the CrRLK1L family, FERONIA. Here we show how MST can also be used to study three-partner interactions.
Language
English
Source (book)
Plant peptide hormones and growth factors
Source (series)
Methods in molecular biology ; 2731
Publication
New York, N.Y. : Humana , 2023
ISBN
978-1-0716-3510-0
DOI
10.1007/978-1-0716-3511-7_21
Volume/pages
p. 279-293
Pubmed ID
38019442
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Project info
Integrating the processes that control cell wall biomechanics during cell growth, using CrRLK1L-regulated root hair growth as a model.
ENDOPOL: endocytosis-regulated cell wall integrity sensing during polarized plant cell growth.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identifier
Creation 12.12.2023
Last edited 13.12.2023
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