Publication
Title
Singlet oxygen-based photoelectrochemical detection of single-point mutations in the KRAS oncogene
Author
Abstract
Single nucleotide point mutations in the KRAS oncogene occur frequently in human cancers, rendering them intriguing targets for diagnosis, early detection and personalized treatment. Current detection methods are based on polymerase chain reaction, sometimes combined with next-generation sequencing, which can be expensive, complex and have limited availability. Here, we propose a novel singlet oxygen (1O2)-based photoelectrochemical detection methodology for single-point mutations, using KRAS mutations as a case study. This detection method combines the use of a sandwich assay, magnetic beads and robust chemical photosensitizers, that need only air and light to produce 1O2, to ensure high specificity and sensitivity. We demonstrate that hybridization of the sandwich hybrid at high temperatures enables discrimination between mutated and wild-type sequences with a detection rate of up to 93.9%. Additionally, the presence of background DNA sequences derived from human cell-line DNA, not containing the mutation of interest, did not result in a signal, highlighting the specificity of the methodology. A limit of detection as low as 112 pM (1.25 ng/mL) was achieved without employing any amplification techniques. The developed 1O2-based photoelectrochemical methodology exhibits unique features, including rapidity, ease of use, and affordability, highlighting its immense potential in the field of nucleic acid-based diagnostics.
Language
English
Source (journal)
Biosensors and bioelectronics. - Barking, 1990, currens
Publication
Barking : 2023
ISSN
0956-5663
DOI
10.1016/J.BIOS.2023.115957
Volume/pages
249 (2024) , p. 1-7
Article Reference
115957
Full text (Publisher's DOI)
Full text (open access)
The author-created version that incorporates referee comments and is the accepted for publication version Available from 23.06.2024
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Project info
Detection and quantification of a panel of clinically relevant DNA biomarker sequences containing KRAS mutations in tissue and liquid biopsies via a novel photoelectrochemical technology.
Molecular design of 'Frozen Aptamers': Aptamers with enhanced properties by covalent and non-covalent stabilization using functionalized nucleotides: a combined modelling, NMR and electrochemistry approach.
Benchmarking singlet oxygen-based photoelectrochemical detection platform for panel analysis of cancer biomarkers, using prostate cancer as a case study. A novel groundbreaking approach for cancer diagnosis and follow-up.
Improving diagnostic accuracy and follow-up of neuroendocrine neoplasms through detection of (epi)genetic biomarkers in liquid biopsies using novel technological platforms.
Liquid biopsies for diagnosis, prognosis and follow-up of neuroendocrine neoplasms: clinical translation of novel technologies for (epi)genetic biomarker detectio.
A new photoelectrochemical singlet oxygen-based detection platform for a panel of cancer biomarkers in tissue and liquid biopsies (SOCAN).
A new photoelectrochemical singlet oxygen-based detection platform for a panel of cancer biomarkers in tissue and liquid biopsies (SOCAN).
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identifier
Creation 03.01.2024
Last edited 11.01.2024
To cite this reference