Publication
Title
Accurate image derived input function in [¹⁸F]SynVesT-1 mouse studies using isoflurane and ketamine/xylazine anesthesia
Author
Abstract
Background Kinetic modeling in positron emission tomography (PET) requires measurement of the tracer plasma activity in the absence of a suitable reference region. To avoid invasive blood sampling, the use of an image derived input function has been proposed. However, an accurate delineation of the blood pool region in the PET image is necessary to obtain unbiased blood activity. Here, to perform brain kinetic modeling in [F-18]SynVesT-1 dynamic scans, we make use of non-negative matrix factorization (NMF) to unmix the activity signal from the different tissues that can contribute to the heart region activity, and extract only the left ventricle activity in an unbiased way. This method was implemented in dynamic [F-18]SynVesT-1 scans of mice anesthetized with either isoflurane or ketamine-xylazine, two anesthestics that we showed to affect differently radiotracer kinetics. The left ventricle activity (NMF-IDIF) and a manually delineated cardiac activity (IDIF) were compared with arterial blood samples (ABS), and for isoflurane anesthetized mice, arteriovenous (AV) shunt blood data were compared as well. Finally, brain regional 2 tissue compartment modeling was performed using IDIF and NMF-IDIF, and the model fit accuracy (weighted symmetrical mean absolute percentage error, wsMAPE) as well as the total volume of distribution (V-T) were compared.Results In isoflurane anesthetized mice, the difference between ABS and NMF-IDIF activity (+ 12.8 +/- 11%, p = 0.0023) was smaller than with IDIF (+ 16.4 +/- 9.8%, p = 0.0008). For ketamine-xylazine anesthetized mice the reduction in difference was larger (NMF-IDIF: 16.9 +/- 10%, p = 0.0057, IDIF: 56.3 +/- 14%, p < 0.0001). Correlation coefficient between isoflurane AV-shunt time activity curves and NMF-IDIF (0.97 +/- 0.01) was higher than with IDIF (0.94 +/- 0.03). The brain regional 2TCM wsMAPE was improved using NMF-IDIF compared with IDIF, in isoflurane (NMF-IDIF: 1.24 +/- 0.24%, IDIF: 1.56 +/- 0.30%) and ketamine-xylazine (NMF-IDIF: 1.40 +/- 0.24, IDIF: 2.62 +/- 0.27) anesthetized mice. Finally, brain V-T was significantly (p < 0.0001) higher using NMF-IDIF compared with IDIF, in isoflurane (3.97 +/- 0.13% higher) and ketamine-xylazine (32.7 +/- 2.4% higher) anesthetized mice.Conclusions Image derived left ventricle blood activity calculated with NMF improves absolute activity quantification, and reduces the error in the kinetic modeling fit. These improvements are more pronounced in ketamine-xylazine than in isoflurane anesthetized mice.
Language
English
Source (journal)
EJNMMI physics / European Association of Nuclear Medicine. - 2014, currens
Publication
2023
ISSN
2197-7364
DOI
10.1186/S40658-023-00599-8
Volume/pages
10 :1 (2023) , p. 1-16
Article Reference
78
ISI
001114347600001
Pubmed ID
38052966
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Project info
Non-invasive motion tracking and motion adaptive resolution modeling for awake rat brain positron emission tomography.
Preclinical PET imaging of allele-selective mHTT lowering as candidate treatment for Huntington's Disease.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 09.01.2024
Last edited 11.01.2024
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