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Title
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Follow-up of humoral immune response after HPV vaccination using first-void urine : a longitudinal cohort study
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Author
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Abstract
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| Assessment of humoral immune responses following human papillomavirus (HPV) vaccination currently relies on invasive blood sampling. This longitudinal cohort study explores the usability of first-void urine as a noninvasive alternative sample for antibody detection. In this study, 58 women receiving three doses of the 9vHPV vaccine within a Gardasil9 (9vHPV) Phase III randomized controlled trial were included. Participants provided paired first-void urine and blood samples before vaccination (M0), 1 month after the third dose (M7), and similar to 3 years after the third dose (M43). Type-specific antibody responses to the 9vHPV types were analyzed in 174 first-void urine and 172 serum samples using a virus-like particle-based IgG multiplex enzyme-linked immunosorbent assay. Additionally, total human IgG concentrations were determined using the BioPlex assay. At M7, 1 month after complete 9vHPV vaccination, 95%-100% of first-void urine and 100% of serum samples had detectable concentrations, varying by HPV type. At M43, 84%-100% of first-void urine and 98%-100% of serum samples had HPV-specific antibody concentrations. Results show significant Spearman rank correlations between type-specific HPV-antibody concentrations for paired first-void urine and serum at all time points. This study confirms the potential feasibility of utilizing first-void urine as a noninvasive immunological sample within HPV vaccine trials. |
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Language
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English
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Source (journal)
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Journal of medical virology. - New York, N.Y.
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Publication
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New York, N.Y.
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2023
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ISSN
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0146-6615
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DOI
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10.1002/JMV.29133
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Volume/pages
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95
:10
(2023)
, p. 1-10
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Article Reference
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e29133
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ISI
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001099616100053
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Pubmed ID
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37812015
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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