Publication
Title
Follow-up of humoral immune response after HPV vaccination using first-void urine : a longitudinal cohort study
Author
Abstract
Assessment of humoral immune responses following human papillomavirus (HPV) vaccination currently relies on invasive blood sampling. This longitudinal cohort study explores the usability of first-void urine as a noninvasive alternative sample for antibody detection. In this study, 58 women receiving three doses of the 9vHPV vaccine within a Gardasil9 (9vHPV) Phase III randomized controlled trial were included. Participants provided paired first-void urine and blood samples before vaccination (M0), 1 month after the third dose (M7), and similar to 3 years after the third dose (M43). Type-specific antibody responses to the 9vHPV types were analyzed in 174 first-void urine and 172 serum samples using a virus-like particle-based IgG multiplex enzyme-linked immunosorbent assay. Additionally, total human IgG concentrations were determined using the BioPlex assay. At M7, 1 month after complete 9vHPV vaccination, 95%-100% of first-void urine and 100% of serum samples had detectable concentrations, varying by HPV type. At M43, 84%-100% of first-void urine and 98%-100% of serum samples had HPV-specific antibody concentrations. Results show significant Spearman rank correlations between type-specific HPV-antibody concentrations for paired first-void urine and serum at all time points. This study confirms the potential feasibility of utilizing first-void urine as a noninvasive immunological sample within HPV vaccine trials.
Language
English
Source (journal)
Journal of medical virology. - New York, N.Y.
Publication
New York, N.Y. : 2023
ISSN
0146-6615
DOI
10.1002/JMV.29133
Volume/pages
95 :10 (2023) , p. 1-10
Article Reference
e29133
ISI
001099616100053
Pubmed ID
37812015
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Project info
Development of a first-void urine based highly sensitive competitive HPV immunoassay.
UROSCREEN: a home-based first-void URine One-step triage option for cervical cancer SCREENing.
Disease and vaccine monitoring based on non-invasive genital tract liquid biopsy sampling (URISAMP).
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 09.01.2024
Last edited 11.01.2024
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