Publication
Title
Myeloid OTULIN deficiency couples RIPK3-dependent cell death to Nlrp3 inflammasome activation and IL-1β secretion
Author
Abstract
Loss-of-function mutations in the deubiquitinase OTULIN result in an inflammatory pathology termed “OTULIN-related autoinflammatory syndrome” (ORAS). Genetic mouse models revealed essential roles for OTULIN in inflammatory and cell death signaling, but the mechanisms by which OTULIN deficiency connects cell death to inflammation remain unclear. Here, we identify OTULIN deficiency as a cellular condition that licenses RIPK3-mediated cell death in murine macrophages, leading to Nlrp3 inflammasome activation and subsequent IL-1β secretion. OTULIN deficiency uncoupled Nlrp3 inflammasome activation from gasdermin D–mediated pyroptosis, instead allowing RIPK3-dependent cell death to act as an Nlrp3 inflammasome activator and mechanism for IL-1β release. Accordingly, elevated serum IL-1β levels in myeloid-specific OTULIN-deficient mice were diminished by deleting either Ripk3 or Nlrp3 . These findings identify OTULIN as an inhibitor of RIPK3-mediated IL-1β release in mice.
Language
English
Source (journal)
Science Immunology
Publication
2023
DOI
10.1126/SCIIMMUNOL.ADF4404
Volume/pages
8 :89 (2023)
Article Reference
eadf4404
Pubmed ID
38000038
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Project info
Dissecting cellular interactions underlying auto-inflammation in Familial Mediterranean Fever.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identifier
Creation 09.01.2024
Last edited 10.01.2024
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