Publication
Title
HILIC UPLC/ QTof MS method development for the quantification of AGEs inhibitors : trouble shooting protocol
Author
Abstract
Objective: The paper reports an attempt to develop and validate a HILIC UPLC/ QTof MS method for quantifying N-ε-carboxymethyl-L-lysine (CML) in vitro, testing N-ε-carboxy- [D2]methyl-L-lysine (d2-CML), and N-ε-carboxy[4,4,5,5-D4]methyl-L-lysine (d4-CML) as internal standards. Method: During the method development, several challenging questions occurred that hindered the successful completion of the method. The study emphasizes the impact of issues, generally overlooked in the development of similar analytical protocols. For instance, the use of glassware and plasticware was critical for the accurate quantification of CML. Moreover, the origin of atypical variation in the response of the deuterated internal standards, though widely used in other experimental procedures, was investigated. Result: A narrative description of the systematic approach used to address the various drawbacks during the analytical method development and validation is presented. Conclusion: Reporting those findings can be considered beneficial while bringing an insightful notion about critical factors and potential interferences. Therefore, some conclusion and ideas can be drawn from these trouble-shooting questions, which might help other researchers to develop more reliable bioanalytical methods, or to raise their awareness of stumbling blocks along the way.
Language
English
Source (journal)
Combinatorial chemistry and high throughput screening : the journal to revolutionize drug discovery.. - Schiphol :
Publication
Schiphol : : Bentham science, , 2023
ISSN
1386-2073
DOI
10.2174/1386207326666230706120451
Volume/pages
26 (2023) , p. 1-15
Full text (Publisher's DOI)
Full text (open access)
The author-created version that incorporates referee comments and is the accepted for publication version Available from 06.05.2024
Full text (publisher's version - intranet only)
UAntwerpen
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Publications with a UAntwerp address
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Record
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Creation 09.01.2024
Last edited 18.01.2024
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