Publication
Title
Transcriptomics of a cytoglobin knockout mouse : insights from hepatic stellate cells and brain
Author
Abstract
The vertebrate respiratory protein cytoglobin (Cygb) is thought to exert multiple cellular functions. Here we studied the phenotypic effects of a Cygb knockout (KO) in mouse on the transcriptome level. RNA sequencing (RNA-Seq) was performed for the first time on sites of major endogenous Cygb expression, i.e. quiescent and activated hepatic stellate cells (HSCs) and two brain regions, hippocampus and hypothalamus. The data recapitulated the up-regulation of Cygb during HSC activation and its expression in the brain. Differential gene expression analyses suggested a role of Cygb in the response to inflammation in HSCs and its involvement in retinoid metabolism, retinoid X receptor (RXR) activation-induced xenobiotics metabolism, and RXR activation-induced lipid metabolism and signaling in activated cells. Unexpectedly, only minor effects of the Cygb KO were detected in the transcriptional profiles in hippocampus and hypothalamus, precluding any enrichment analyses. Furthermore, the transcriptome data pointed at a previously undescribed potential of the Cygb- knockout allele to produce cis-acting effects, necessitating future verification studies.
Language
English
Source (journal)
Journal of inorganic biochemistry. - New York, N.Y.
Publication
New York, N.Y. : 2024
ISSN
0162-0134
DOI
10.1016/J.JINORGBIO.2023.112405
Volume/pages
250 (2024) , p. 1-18
Article Reference
112405
ISI
001134611000001
Pubmed ID
37977965
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 01.02.2024
Last edited 05.02.2024
To cite this reference