Publication
Title
Effect of accelerated high-fluence riboflavin and rose bengal-mediated corneal cross-linking on resistance to enzymatic digestion
Author
Abstract
PurposeThis study evaluated the effect of high-fluence accelerated corneal cross-linking on the resistance to enzymatic digestion, assessing two chromophore/light combinations: riboflavin/UV-A light (RF/UV-A) and rose bengal/green light (RB/green).MethodsFreshly prepared ex-vivo porcine corneas (n = 189) were divided into 8 groups groups. Group A corneas were unirradiated controls without chromophore soaking (A0), or soaked with riboflavin (A1) or rose bengal (A2). Group B corneas underwent accelerated epi-off RF/UV-A CXL at fluences of 5.4 J/cm(2) (B1), 10 J/cm(2) (B2), or 15 J/cm(2) (B3). Group C corneas underwent accelerated epi-off RB/green CXL at fluences of either 10 J/cm(2) (C1) or 15 J/cm(2) (C2). Following CXL, all corneas were digested in 0.3% collagenase-A solution, and the time until complete dissolution was measured.ResultsNon-irradiated controls exposed to RF and RB enhanced corneal resistance to collagenase digestion, with RB having a stronger effect than RF. RF/UV-A-treated corneas showed significantly increased digestion resistance with increasing fluence levels. RB/green-treated corneas displayed enhanced digestion resistance with each increase in fluence up to 10 J/cm(2); a 15 J/cm(2) fluence yielded similar digestion resistance times to a 10 J/cm(2) fluence, suggesting a plateau effect in accelerated RB/green CXL protocols.ConclusionsWhen compared to standard-fluence treatments, high-fluence accelerated epi-off CXL using both riboflavin and rose bengal significantly increases resistance to enzymatic digestion. The optimal settings for clinical protocols might be 15 J/cm(2) (30 mW/cm(2) for 8 min 20 s) for RF/UV-A and 10 J/cm(2) (15 mW/cm(2) for 11 min 7 s) for RB/Green Light.
Language
English
Source (journal)
BMC ophthalmology. - London
Publication
London : 2024
ISSN
1471-2415
DOI
10.1186/S12886-024-03293-0
Volume/pages
24 :1 (2024) , p. 1-7
Article Reference
37
ISI
001150524900004
Pubmed ID
38267904
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 04.03.2024
Last edited 08.12.2024
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