Publication
Title
In vitro modelling of bacterial pneumonia : a comparative analysis of widely applied complex cell culture models
Author
Abstract
Bacterial pneumonia greatly contributes to the disease burden and mortality of lower respiratory tract infections among all age groups and risk profiles. Therefore, laboratory modelling of bacterial pneumonia remains important for elucidating the complex host–pathogen interactions and to determine drug efficacy and toxicity. In vitro cell culture enables for the creation of high-throughput, specific disease models in a tightly controlled environment. Advanced human cell culture models specifically, can bridge the research gap between the classical two-dimensional cell models and animal models. This review provides an overview of the current status of the development of complex cellular in vitro models to study bacterial pneumonia infections, with a focus on air–liquid interface models, spheroid, organoid, and lung-on-a-chip models. For the wide scale, comparative literature search, we selected six clinically highly relevant bacteria (Pseudomonas aeruginosa, Mycoplasma pneumoniae, Haemophilus influenzae, Mycobacterium tuberculosis, Streptococcus pneumoniae, and Staphylococcus aureus). We reviewed the cell lines that are commonly used, as well as trends and discrepancies in the methodology, ranging from cell infection parameters to assay read-outs. We also highlighted the importance of model validation and data transparency in guiding the research field towards more complex infection models.
Language
English
Source (journal)
FEMS microbiology: reviews / Federation of European Microbiological Societies. - Amsterdam
Publication
Amsterdam : 2024
ISSN
0168-6445 [print]
1574-6976 [online]
DOI
10.1093/FEMSRE/FUAE007
Volume/pages
48 :2 (2024) , p. 1-23
Article Reference
fuae007
ISI
001178531300001
Pubmed ID
38409952
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Project info
Turning black into golden soldier fly larvae (Hermetia Illucens): added value creation by exploring its microbiota and metabolism (ETOBIOTA).
Turning black into golden soldier fly larvae (Hermetia Illucens): added value creation by exploring its microbiota and metabolism (ETOBIOTA).
Development of a comprehensive platform for targeting redox homeostasis in Mycobacterium tuberculosis.
In vivo multidrug-tolerant persister cells and their evolution in the face of antibiotic treatment: genetic and physiological adaptation mechanisms.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identifier
Creation 06.03.2024
Last edited 02.04.2024
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