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Title
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The underappreciated diversity of bile acid modifications
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Author
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Abstract
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| The repertoire of modifications to bile acids and related steroidal lipids by host and microbial metabolism remains incompletely characterized. To address this knowledge gap, we created a reusable resource of tandem mass spectrometry (MS/MS) spectra by filtering 1.2 billion publicly available MS/MS spectra for bile-acid-selective ion patterns. Thousands of modifications are distributed throughout animal and human bodies as well as microbial cultures. We employed this MS/MS library to identify polyamine bile amidates, prevalent in carnivores. They are present in humans, and their levels alter with a diet change from a Mediterranean to a typical American diet. This work highlights the existence of many more bile acid modifications than previously recognized and the value of leveraging public large-scale untargeted metabolomics data to discover metabolites. The availability of a modification-centric bile acid MS/MS library will inform future studies investigating bile acid roles in health and disease. |
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Language
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English
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Source (journal)
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Cell. - Cambridge, Mass.
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Publication
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Cambridge, Mass.
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2024
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ISSN
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0092-8674
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DOI
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10.1016/J.CELL.2024.02.019
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Volume/pages
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187
:7
(2024)
, p. 1801-1818.e20
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ISI
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001220763300001
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Pubmed ID
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38471500
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Full text (Publisher's DOI)
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Full text (open access)
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