Publication
Title
Integrated staging of morphofunctional connectivity in neuronal cultures
Author
Abstract
Neurological disorders are defined by synaptic dysfunction. We present a workflow to quantify morphological and functional aspects of synaptic connectivity in neuronal cultures and obtain an integrated readout. We describe steps for measuring synchronous calcium bursting in GCaMP6f-transduced neurons and labeling mature synapses using a proximity ligation assay. The integration of functional and morphological information from the same cultures provides a rich fingerprint of synaptic connectivity, deployable in different experimental conditions.
Language
English
Source (journal)
STAR protocols
Publication
2024
DOI
10.1016/J.XPRO.2024.102957
Volume/pages
5 :2 (2024) , p. 1-25
Article Reference
102957
Pubmed ID
38492228
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identifier
Creation 21.03.2024
Last edited 22.03.2024
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