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Title
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Integrated staging of morphofunctional connectivity in neuronal cultures
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Author
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Abstract
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| Neurological disorders are defined by synaptic dysfunction. We present a workflow to quantify morphological and functional aspects of synaptic connectivity in neuronal cultures and obtain an integrated readout. We describe steps for measuring synchronous calcium bursting in GCaMP6f-transduced neurons and labeling mature synapses using a proximity ligation assay. The integration of functional and morphological information from the same cultures provides a rich fingerprint of synaptic connectivity, deployable in different experimental conditions. |
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Language
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English
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Source (journal)
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STAR protocols
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Related dataset(s)
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Publication
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2024
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DOI
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10.1016/J.XPRO.2024.102957
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Volume/pages
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5
:2
(2024)
, p. 1-25
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Article Reference
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102957
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ISI
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001224786600001
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Pubmed ID
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38492228
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Full text (Publisher's DOI)
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Full text (open access)
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