Title
|
|
|
|
Integrated staging of morphofunctional connectivity in neuronal cultures
|
|
Author
|
|
|
|
|
|
Abstract
|
|
|
|
Neurological disorders are defined by synaptic dysfunction. We present a workflow to quantify morphological and functional aspects of synaptic connectivity in neuronal cultures and obtain an integrated readout. We describe steps for measuring synchronous calcium bursting in GCaMP6f-transduced neurons and labeling mature synapses using a proximity ligation assay. The integration of functional and morphological information from the same cultures provides a rich fingerprint of synaptic connectivity, deployable in different experimental conditions. |
|
|
Language
|
|
|
|
English
|
|
Source (journal)
|
|
|
|
STAR protocols
|
|
Related dataset(s)
|
|
|
|
|
|
Publication
|
|
|
|
2024
|
|
DOI
|
|
|
|
10.1016/J.XPRO.2024.102957
|
|
Volume/pages
|
|
|
|
5
:2
(2024)
, p. 1-25
|
|
Article Reference
|
|
|
|
102957
|
|
ISI
|
|
|
|
001224786600001
|
|
Pubmed ID
|
|
|
|
38492228
|
|
Full text (Publisher's DOI)
|
|
|
|
|
|
Full text (open access)
|
|
|
|
|
|