Publication
Title
Investigation of in vitro biotransformation of tris (1-chloro-2-propyl) phosphate and confirmation in human urine
Author
Abstract
Tris (1-chloro-2-propyl) phosphate (TCIPP) is one of the major organophosphate flame retardants present in the indoor and outdoor environment. Knowledge of biotransformation pathways is important to elucidate potential bioavailability and toxicity of TCIPP and to identify relevant biomarkers. This study aimed to identify TCIPP metabolites through in vitro human metabolism assays and finally to confirm these findings in urine samples from an occupationally exposed population to propose new biomarkers to accurately monitor exposure to TCIPP. TCIPP was incubated with human liver microsomes and human liver cytosol to identify Phase I and Phase II metabolites, by liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). Using a suspect-screening approach, the established biomarkers bis (1-chloro-2-propyl) hydrogen phosphate (BCIPP) and 1-hydroxy-2-propyl bis (1-chloro-2-propyl) phosphate (BCIPHIPP) were identified. In addition, carboxyethyl bis (1-chloro-2-propyl) phosphate (TCIPP-M1), bis (1-chloropropan-2-yl) (-oxopropan-2-yl) phosphate (TCIPP-M2) and 1-chloro-3-hydroxypropan-2-yl bis (1-chloropropan-2-yl) phosphate (TCIPP-M3) were identified. TCIPP-M2, an intermediate product, was not reported before in literature. In urine samples, apart from BCIPP and BCIPHIPP, TCIPP-M1 and TCIPP-M3 were identified for the first time. Interestingly, BCIPP showed the lowest detection frequency, likely due to the poor sensitivity for this compound. Therefore, TCIPP-M1 and TCIPP-M3 could serve as potential additional biomarkers to more efficiently monitor TCIPP exposure in humans.
Language
English
Source (journal)
Current research in toxicology. - [Amsterdam], 2020, currens
Publication
[Amsterdam] : Elsevier B.V , 2024
ISSN
2666-027X
DOI
10.1016/J.CRTOX.2024.100164
Volume/pages
6 (2024) , p. 1-11
Article Reference
100164
ISI
001218117600001
Pubmed ID
38550635
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Project info
Novel mass-spectrometric approaches to investigate human exposure to emerging contaminants.
Novel analytical and environmental insights in the human exposure to Synthetic ANTioxidants by Advanced mass spectrometric workflows (SANTA).
From exposome to effect assessment of contaminants in human and animal models (EXPOSOME).
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 28.03.2024
Last edited 07.11.2024
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