Publication
Title
HPV-specific antibodies in female genital tract secretions captured via first-void urine retain their neutralizing capacity
Author
Abstract
Human papillomavirus (HPV) vaccines, primarily relying on neutralizing antibodies, have proven highly effective. Recently, HPV-specific antibodies have been detected in the female genital tract secretions captured by first-void urine (FVU), offering a minimally invasive diagnostic approach. In this study, we investigated whether HPV16-specific antibodies present in FVU samples retain their neutralizing capacity by using pseudovirion-based neutralization assays. Paired FVU and serum samples (vaccinated n = 25, unvaccinated n = 25, aged 18–25) were analyzed using two orthogonal pseudovirion-based neutralization assays, one using fluorescence microscopy and the other using luminescence-based spectrophotometry. Results were compared with HPV16-specific IgG concentrations and correlations between neutralizing antibodies in FVU and serum were explored. The study demonstrated the presence of neutralizing antibodies in FVU using both pseudovirion-based neutralization assays, with the luminescence-based assay showing higher sensitivity for FVU samples, while the fluorescence microscopy-based assay exhibited better specificity for serum and overall higher reproducibility. High Spearman correlation values were calculated between HPV16-IgG and HPV16-neutralizing antibodies for both protocols (rs: 0.54–0.94, p < .001). Significant Spearman correlations between FVU and serum concentrations were also established for all assays (rs: 0.44–0.91, p < .01). This study demonstrates the continued neutralizing ability of antibodies captured with FVU, supporting the hypothesis that HPV vaccination may reduce autoinoculation and transmission risk to the sexual partner. Although further protocol optimizations are warranted, these findings provide a foundation for future research and larger cohort studies that could have implications for the optimal design, evaluation, and implementation of HPV vaccination programs.
Language
English
Source (journal)
Human vaccines & immunotherapeutics. - Philadelphia, Pa, 2012, currens
Publication
Philadelphia, Pa : 2024
ISSN
2164-5515 [print]
2164-554X [online]
DOI
10.1080/21645515.2024.2330168
Volume/pages
20 :1 (2024) , p. 1-12
Article Reference
2330168
ISI
001196409200001
Pubmed ID
38567541
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Project info
Development of a first-void urine based highly sensitive competitive HPV immunoassay.
Disease and vaccine monitoring based on non-invasive genital tract liquid biopsy sampling (URISAMP).
Border control: Exposing the molecular mechanisms of nuclear envelope rupture and repair
Unravelling the cellular response to photoporation.
Interactive and intelligent cellomics platform.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identifier
Creation 17.04.2024
Last edited 06.05.2024
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