Publication
Title
Singlet oxygen-based photoelectrochemical detection of miRNAs in prostate cancer patients’ plasma : a novel diagnostic tool for liquid biopsy
Author
Abstract
Dysregulation of miRNA expression occurs in many cancers, making miRNAs useful in cancer diagnosis and therapeutic guidance. In a clinical context using methods such as polymerase chain reaction (PCR), the limited amount of miRNAs in circulation often limits their quantification. Here, we present a PCR-free and sensitive singlet oxygen (1O2)-based strategy for the detection and quantification of miRNAs in untreated human plasma from patients diagnosed with prostate cancer. A target miRNA is specifically captured by functionalised magnetic beads and a detection oligonucleotide probe in a sandwich-like format. The formed complex is concentrated at the sensor surface via magnetic beads, providing an interface for the photoinduced redox signal amplification. The detection oligonucleotide probe bears a molecular photosensitiser, which produces 1O2 upon illumination, oxidising a redox reporter and creating a redox cycling loop, allowing quantification of pM level miRNA in diluted human plasma within minutes after hybridisation and without target amplification.
Language
English
Source (journal)
Bioelectrochemistry: an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry. - Oxford
Publication
Oxford : Elsevier , 2024
ISSN
1567-5394
DOI
10.1016/J.BIOELECHEM.2024.108698
Volume/pages
158 (2024) , p. 1-9
Article Reference
108698
Pubmed ID
38640856
Full text (Publisher's DOI)
Full text (open access)
The author-created version that incorporates referee comments and is the accepted for publication version Available from 18.10.2024
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Project info
A new photoelectrochemical singlet oxygen-based detection platform for a panel of cancer biomarkers in tissue and liquid biopsies (SOCAN).
A new photoelectrochemical singlet oxygen-based detection platform for a panel of cancer biomarkers in tissue and liquid biopsies (SOCAN).
A new photoelectrochemical singlet oxygen-based detection platform for a panel of cancer biomarkers in tissue and liquid biopsies (SOCAN).
Innovative electrochemical multiplex biosensor for detection and quantification of clinically relevant circulating miRNAs (INFORM).
Molecular design of 'Frozen Aptamers': Aptamers with enhanced properties by covalent and non-covalent stabilization using functionalized nucleotides: a combined modelling, NMR and electrochemistry approach.
A novel rolling circle amplification-mediated photoelectrochemical detection methodology for arboviruses (ArboSense).
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identifier
Creation 24.04.2024
Last edited 25.04.2024
To cite this reference