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Title
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Phytochemical and Antidiabetic Investigations on Marine Natural Products from Indonesia = Fytochemisch en Antidiabetisch Onderzoek van Mariene Natuurproducten uit Indonesië
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Author
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Abstract
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| Diabetes mellitus is a metabolic disorder characterized by elevated blood glucose levels. Controlling the fluctuation of blood glucose levels by inhibiting α-glucosidase activity is one possible strategy of diabetes management. Marine organisms, such as sea cucumbers and mangrove plants, are important resources of natural products. This current project was therefore intended to investigate the phytochemical compounds present in the body wall of black sea cucumber (H. atra) and mangrove (R. mucronata) leaves, respectively; and to determine the α-glucosidase inhibitory activity from the body wall of black sea cucumber (H. atra) and mangrove (R. mucronata) leaves by in vitro assays. Phytochemical investigations of a body wall sub-fraction of black sea cucumber (H. atra) resulted in two isolated compounds: one previously reported triterpene glycoside, desholothurin B, and one triterpene glycoside isolated for the first time, 12-epi-desholothurin B. According to the UPLC-HRMS analysis, several compounds have been identified in black sea cucumber (H. atra), including 17-hydroxyfuscocineroside B (= scabraside B), 25-hydroxyfuscocineroside B, leucospilotaside A, holothurin A3, holothurin A5 (II), holothurin A1, holothurin A4, scabraside D, 17-dehydroxyholothurin A (= fuscocineroside C), scabraside A, calcigeroside B, 24-dehydroechinoside A, fuscocineroside B, holothurin B2, holothurin B, holothurin B4, nobiliside II (= ananaside C), nobiliside B (I), nobiliside C (II) (= nobiliside B), leucospilatoside C, echinoside A (= holothurin A2), holothurin B3 (24-dehydroechinoside B), echinoside B (= holothurin B1), 4 of which have not been reported in H. atra body wall before. The IC50 values of the 90% methanolic fraction, of the 90% methanolic subfraction and of acarbose were 0.158±0.002 mg/mL, 0.181±0.006 mg/mL and 2.340±0.044 mg/mL, respectively. The study on R. mucronata mangrove leaves showed that four phytochemical compounds including rutin, tetrahydroxyflavone-O-hexoside, tetrahydroxyflavone-O-deoxyhexose-O-hexoside, and tetrahydroxy-methoxyflavone-O-deoxyhexose-O-hexoside are present in unfermented tea, the crude methanolic extract and in kombucha tea. The α-glucosidase inhibitory activity of R. mucronata leaves of the unfermented tea powder (IC50 = 0.123±0.023 mg/mL) and of the crude methanolic extract 80% (IC50 = 0.046±0.001 mg/mL) were reported for the first time. The IC50 value of acarbose (2.40±0.20 mg/mL) showed lower activity than unfermented tea powder and crude 80% methanolic extract in inhibiting α-glucosidase activity. These findings indicate that the phytochemical compounds of the body wall of black sea cucumber (H. atra) and mangrove (R. mucronata) leaves show α-glucosidase inhibitory properties. On that note, the results of this study may contribute to the development of food supplements or pharmaceuticals based on marine natural products. |
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Language
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English
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Publication
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Antwerp - Malang
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University of Antwerp & Graduate School of Universitas Brawijaya
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2024
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DOI
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10.63028/10067/2079460151162165141
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Volume/pages
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257 p.
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Note
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Hermans, Nina [Supervisor]
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de Bruyne, Tess [Supervisor]
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Aulanni'am, A. [Supervisor]
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Hariati, Anik M. [Supervisor]
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Full text (open access)
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