Publication
Title
Bulk RNA-seq analysis of HepG2 exposed to oleic and palmitic acid
Author
Abstract
We hypothesized that exposure to fatty acids in metabolic dysfunction-associated steatohepatitis-like environment will profoundly affect gene expression of hepatocytes. More precisely, we wish to investigate expression of genes related to ferroptosis, i.e. an iron-catalyzed form of cell death through lethal lipid peroxidation. We aimed to study the effect of fatty acid supplementation in a metabolic dysfunction-associated steatohepatitis-like environment on gene expression of HepG2 cells profiled with bulk mRNA-sequencing. HepG2 cells were exposed for 48 hours to oleic acid (100microM) and palmitic acid (50microM), as well as hyperglycemia (4.5 mg/mL), hyperinsulinemia (100 nM), tumor necrosis factor alpha (50 ng/mL), interleukin 1-beta (25 ng/mL) and transforming growth factor-beta (8ng/mL). Control samples were exposed to solvents needed to dissolve oleic acid and palmitic acid in medium.
Language
English
Related publication(s)
Publication
NCBI - GEO , 2024
Volume/pages
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Source file
Record
Identifier c:irua:208072
Creation 23.09.2024
Last edited 24.09.2024
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