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Title
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Improved sample preparation method for selected persistent organochlorine pollutants in human serum using solid-phase disk extraction with gas chromatographic analysis
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Author
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Abstract
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| An improved solid-phase extraction (SPE) method was developed to isolate and concentrate trace levels of selected POPs (persistent organochlorine pollutants) in human serum prior to GC-NIS in SIM mode or GC-ECD quantitation. The extraction involves denaturation of serum proteins with formic acid, SPE using C-18 Empore(TM) disk cartridges, followed by elimination of lipid interferences using a sulfuric acid wash of the eluate. Use of the SPE disk improved assay throughput and gave a cleaner analytical matrix compared with previously reported solid-phase and liquid-liquid extraction techniques. The extraction method provided consistent recoveries at three fortification levels using C-13(12) PCB 149 as internal standard. Recoveries ranged from 48 to 140% for organochlorine pesticides (6.25, 12.5 and 25 ng/ml) and 71 to 126% for polychlorinated biphenyls (0.625, 1.25 and 2.5 ng/ml). (C) 1999 Elsevier Science B.V. All rights reserved. |
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Language
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English
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Source (journal)
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Journal of chromatography : biomedical applications. - Amsterdam, 1977 - 1993
Journal of chromatography : B: biomedical sciences and applications. - Amsterdam, 1997 - 2001
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Publication
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Amsterdam
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Elsevier
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1999
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ISSN
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0378-4347
[print]
1872-812X
[online]
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Volume/pages
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723
:1-2
(1999)
, p. 117-125
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ISI
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000078700800013
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Full text (Publisher's DOI)
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