Title
A novel disorder caused by defective biosynthesis of N-linked oligosaccharides due to glucosidase I deficiency A novel disorder caused by defective biosynthesis of N-linked oligosaccharides due to glucosidase I deficiency
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
New York, N.Y. ,
Source (journal)
The American journal of human genetics. - New York, N.Y.
Volume/pages
66(2000) , p. 1744-1756
ISSN
0002-9297
ISI
000088373800003
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Glucosidase I is an important enzyme in N-linked glycoprotein processing, removing specifically distal α-1,2linked glucose from the Glc3Man9GlcNAc2 precursor after its en bloc transfer from dolichyl diphosphate to a nascent polypeptide chain in the endoplasmic reticulum. We have identified a glucosidase I defect in a neonate with severe generalized hypotonia and dysmorphic features. The clinical course was progressive and was characterized by the occurrence of hepatomegaly, hypoventilation, feeding problems, seizures, and fatal outcome at age 74 d. The accumulation of the tetrasaccharide Glc(α1-2)Glc(α1-3)Glc(α1-3)Man in the patient's urine indicated a glycosylation disorder. Enzymological studies on liver tissue and cultured skin fibroblasts revealed a severe glucosidase I deficiency. The residual activity was <3% of that of controls. Glucosidase I activities in cultured skin fibroblasts from both parents were found to be 50% of those of controls. Tissues from the patient subjected to SDS-PAGE followed by immunoblotting revealed strongly decreased amounts of glucosidase I protein in the homogenate of the liver, and a less-severe decrease in cultured skin fibroblasts. Molecular studies showed that the patient was a compound heterozygote for two missense mutations in the glucosidase I gene: (1) one allele harbored a G→C transition at nucleotide (nt) 1587, resulting in the substitution of Arg at position 486 by Thr (R486T), and (2) on the other allele a T→C transition at nt 2085 resulted in the substitution of Phe at position 652 by Leu (F652L). The mother was heterozygous for the G→C transition, whereas the father was heterozygous for the T→C transition. These base changes were not seen in 100 control DNA samples. A causal relationship between the α-glucosidase I deficiency and the disease is postulated.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/11b74f/5653.pdf
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