Publication
Title
Binding of mineral elements to locust bean gum influences availability in vitro
Author
Abstract
Experiments were conducted to evaluate the extent to which element binding of locust bean gum (LBG) affects the availability of calcium, iron, and zinc in the gut. Infant formula was supplemented with increasing amounts of LBG and subjected to an intraluminal digestion procedure. Element binding was measured by eliminating the complexes by twofold centrifugation. Availability of the elements was determined using a validated continuous-flow dialysis technique. Elemental content of the samples, supernatants, and dialysates was analyzed with validated atomic absorption spectrometry. LBG provided small amounts of intrinsic calcium (1.13 +/- 0.02 mg/g) and trace amounts of iron (0.02 +/- 0.00 mg/g) and zinc (0.01 +/- 0.00 mg/g), which were strongly bound to the LBG molecule (respectively: 76.6 +/- 3.3%, 83.4 +/- 1.2%, 96.7 +/- 6.6%). Correlation analysis, between percent element bound by LBG after centrifugation and percent trapped after dialysis, yielded significant correlation only for the data of zinc (r = 0.93). For calcium and iron, no correlation could be demonstrated, however, for iron a similar trend was observed. These findings suggest that element binding of LBG has a major influence on the availability of zinc and maybe of iron. For calcium, other factors might also be involved, affecting availability.
Language
English
Source (journal)
Biological trace element research. - London
Publication
London : 2001
ISSN
0163-4984
Volume/pages
81 :1 (2001) , p. 79-92
ISI
000170248400007
Pubmed ID
11508334
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 08.10.2008
Last edited 05.12.2021
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