Title
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Short capillary ion-pair high performance LC-MS coupled to ESMSMS for the simultaneous analysis of nucleoside mono-, di and triphosphates
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Author
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Abstract
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A liquid chromatography/mass spectrometry (LC/MS) method for the analysis of complex mixtures of nucleoside mono-, di- and triphosphates has been developed. A short capillary column (35mm x 0.3mm i.d.) was operated under ion-pair high-performance liquid chromatography conditions and hyphenated to (negative) electrospray (tandem) mass spectrometry. As such, the separation of 12 nucleotides was performed by a binary gradient elution using CH3OH/H2O and N,N-dimethylhexylamine (N,N-DMHA) as ion-pairing agent. The influence of different N,N-DMHA concentrations on the chromatographic and mass spectrometric performance was evaluated to achieve optimal LC/MS conditions. In addition it was demonstrated that a controlled admission of ammonium dihydrogen phosphate (NH4H2PO4) improved both chromatographic performance and mass spectrometric detection. Because the system was hyphenated to an orthogonal designed electrospray interface (Z-spray(TM)), long acquisition times were possible without loss of sensitivity. Copyright (C) 2002 John Wiley Sons, Ltd. |
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Language
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English
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Source (journal)
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Rapid communications in mass spectrometry. - London
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Publication
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London
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2002
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ISSN
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0951-4198
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DOI
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10.1002/RCM.704
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Volume/pages
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16
:12
(2002)
, p. 1205-1215
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ISI
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000176158900012
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Full text (Publisher's DOI)
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