Publication
Title
Short capillary ion-pair high performance LC-MS coupled to ESMSMS for the simultaneous analysis of nucleoside mono-, di and triphosphates
Author
Abstract
A liquid chromatography/mass spectrometry (LC/MS) method for the analysis of complex mixtures of nucleoside mono-, di- and triphosphates has been developed. A short capillary column (35mm x 0.3mm i.d.) was operated under ion-pair high-performance liquid chromatography conditions and hyphenated to (negative) electrospray (tandem) mass spectrometry. As such, the separation of 12 nucleotides was performed by a binary gradient elution using CH3OH/H2O and N,N-dimethylhexylamine (N,N-DMHA) as ion-pairing agent. The influence of different N,N-DMHA concentrations on the chromatographic and mass spectrometric performance was evaluated to achieve optimal LC/MS conditions. In addition it was demonstrated that a controlled admission of ammonium dihydrogen phosphate (NH4H2PO4) improved both chromatographic performance and mass spectrometric detection. Because the system was hyphenated to an orthogonal designed electrospray interface (Z-spray(TM)), long acquisition times were possible without loss of sensitivity. Copyright (C) 2002 John Wiley Sons, Ltd.
Language
English
Source (journal)
Rapid communications in mass spectrometry. - London
Publication
London : 2002
ISSN
0951-4198
Volume/pages
16:12(2002), p. 1205-1215
ISI
000176158900012
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 08.10.2008
Last edited 15.06.2017
To cite this reference