Title
Genotoxicity of melanoidin fractions derived from a standard glucose/glycine model Genotoxicity of melanoidin fractions derived from a standard glucose/glycine model
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Washington, D.C. ,
Subject
Chemistry
Biology
Pharmacology. Therapy
Source (journal)
Journal of agricultural and food chemistry. - Washington, D.C.
Volume/pages
52(2004) :2 , p. 318-323
ISSN
0021-8561
0021-8561
ISI
000188366300028
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Genotoxic compounds can act at various levels in the cell (causing gene, chromosome, or genome mutations), necessitating the use of a range of genotoxicity assays designed to detect these different types of. mutations. The production of melanoidins during the processing and cooking of foods is associated with changes in their nutritional character, and the discovery of mutagenic substances in pyrolyzed protein and amino acids has raised concern about the safety of these foods. The aim of this work was to test melanoidin fractions in three different in vitro assays (Ames test, Vitotox test, and micronucleus test). These melanoidin fractions were produced from the condensation of glucose with glycine and their separation was conducted by dialysis. The crude reaction mixture (before dialysis) and both the LMW and HMW. fractions obtained by dialysis showed no genotoxicity in these assays, despite being tested at concentrations much higher than those naturally found in food products. The LMW fraction, however, showed toxicity at these high concentrations. The volatile fraction produced in this reaction showed genotoxicity only in the Vitotox test, at high concentrations.
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