Title
Effects of interleukin-4 (IL-4) on myelopoiesis : studies on highly purified <tex>$CD34^{+}$</tex> hematopoietic progenitor cells Effects of interleukin-4 (IL-4) on myelopoiesis : studies on highly purified <tex>$CD34^{+}$</tex> hematopoietic progenitor cells
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Baltimore, Md ,
Subject
Human medicine
Source (journal)
Leukemia. - Baltimore, Md, 1987, currens
Volume/pages
7(1993) :4 , p. 625-629
ISSN
0887-6924
1476-5551
ISI
A1993KY97300020
Carrier
E
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
We studied the effects of interleukin 4 (IL4) on myelopoiesis supported by either granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage CSF (GM-CSF) or IL3 on purified CD34 + bone marrow progenitor cells. IL4 stimulates the colony-forming unit granulocyte (CFU-G) induced by G-CSF and inhibits all colony types supported by either IL3 and GM-CSF, although inhibition of CFU-M (macrophage) was significantly stronger than that of CFU-G and CFU-GM. When the cells were first incubated in liquid culture for 4 days in IL4, followed by agar culture in G-CSF, there was a significant increase in the number of CFU-G compared to cells which had been incubated in medium alone for 4 days before plating out in agar containing G-CSF. The inhibitory effects of IL4 on GM-CSF or IL3 supported colony formation, however, disappear with sequential incubation in IL4 in liquid culture followed by culture in agar with either GM-CSF or IL3.
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