Title
Differential regulation of the expression of CD38 and HLA-DR on <tex>$CD34^{+}$</tex> hematopoietic progenitor cells by interleukin-4 and interferon-gamma Differential regulation of the expression of CD38 and HLA-DR on <tex>$CD34^{+}$</tex> hematopoietic progenitor cells by interleukin-4 and interferon-gamma
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Oak Ridge, Tenn. ,
Subject
Human medicine
Source (journal)
Experimental hematology. - Oak Ridge, Tenn., 1973, currens
Volume/pages
21(1993) :11 , p. 1480-1486
ISSN
0301-472X
1873-2399
ISI
A1993MW61700017
Carrier
E
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
We studied the effects of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) on the expression of CD38 and human leukocyte antigen (HLA)-DR on purified CD34(+) bone marrow progenitor cells. CD34(+)CD38(-) and CD34(+)HLA-DR(-) cells are largely nonoverlapping populations. After culture for 4 days in IFN-gamma, the expression of CD38 and HLA-DR is significantly increased and the disappearance of the CD38(-) and HLA-DR(-) populations is virtually complete. Moreover, IFN-gamma induces a population of CD34(+) tells with a very high expression of CD38 (CD34(+)CD38(++) cells), which were absent in the initial CD34(+) population. IL-4 has no effect on the expression of CD38, but induces a limited but significant increase in the expression of HLA-DR. After culture in IFN-gamma, CD34(+) cells show a higher cloning efficiency of the colony-forming unit-macrophage (CFU-M) and burst-forming unit-erythroid (BFU-E) compared to cells cultured in medium alone. After culture in IL-4, a limited increase in CFU-granulocyte (CFU-G) and BFU-E is seen, whereas CFU-G, CFU-M, and BFU-E are increased after culture in IL-4 plus IFN-gamma. We further investigated the functional properties of the CD34(+)CD38(++) cells generated in the presence of IFN-gamma. This cell population is highly enriched for BFU-E but partially depleted of CFU-M. Most of the CFU-M were found in the CD34(+)CD38(+)/(-) (CD34(+)CD38(-) and CD34(+)CD38(+) cells) population.
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