Title
Effects of tyrosine kinase and phosphatase inhibitors on microtubules in Arabidopsis root cells Effects of tyrosine kinase and phosphatase inhibitors on microtubules in Arabidopsis root cells
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
London ,
Source (journal)
Cell biology international. - London, 1993, currens
Volume/pages
32(2008) :6 , p. 630-637
ISSN
1065-6995
1095-8355
ISI
000260671300005
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
To investigate the role of tyrosine phosphorylation/dephosphorylation processes in plant cells the morphology of Arabidopsis thaliana primary roots and the organization of cortical microtubules (MTs) were studied after inhibition of protein tyrosine kinases (PTKs) and tyrosine phosphatases (PTPs). It was found that all tested types of PTKs inhibitors (herbimycin A, genistein and tyrphostin AG 18) altered root hair growth and development, probably as a result of their significant influences on MTs organization in root hairs. The treatment also led to MTs reorientation and disruption in epidermis and cortex cells of both elongation and differentiation zones of primary roots. Enhanced tyrosine phosphorylation after treatment with a PTPs inhibitor (sodium orthovanadate) resulted in intense induction of root hair development and growth and caused a significant shortening of the elongation zone. It also led to changes of MTs orientation from transverse to longitudinal in epidermis and cortex cells of the elongation and differentiation zones of the root. From the data obtained we can suppose that tyrosine phosphorylation can be involved in the dynamics and organization of MTs in different types of plant cells.
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