Title
The combination of resonance Raman spectroscopy, optical tweezers and microfluidic systems applied to the study of various heme-containing single cellsThe combination of resonance Raman spectroscopy, optical tweezers and microfluidic systems applied to the study of various heme-containing single cells
Author
Faculty/Department
Faculty of Sciences. Physics
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Research group
Biophysics and Biomedical Physics
Experimental condensed matter physics (ECM)
Proteinchemistry, proteomics and epigenetic signalling(PPES)
VIB DMG - Applied Molecular Genomics
Publication type
article
Publication
Source (journal)
Spectroscopy: an international journal
Volume/pages
22(2008):4, p. 287-295
ISI
000258077600008
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Several recent studies on the function of neuroglobin (Ngb), a hemoprotein predominantly expressed in the brain, point toward a neuro-protective role during hypoxic-ischemic injuries. The exact mechanism by which Ngb protects the cell against H<sub>2</sub>O<sub>2</sub>-induced cell death remains to be elucidated. Hence, new tools need to be developed in order to study the protein in vivo or under physiological conditions. In this summary of our work, we demonstrate how resonance Raman spectroscopy, optical tweezers and microfluidic systems were combined to mimic in vivo conditions in an in vitro milieu. The setup has been tested on several globin-containing cells: hemoglobin (Hb) within single red blood cells (RBCs), a nerve globin present in the nerve cord of the annelid Aphrodite aculeata (A. aculeata), and wild-type (wt) human neuroglobin (NGB) overexpressed in Escherichia coli (E. coli) bacteria. The feasibility of the setup regarding sensitivity and photo-induced effects and the results regarding the oxygen uptake and release will be discussed and compared for each system. The summary of the results show that the method is promising and the setup will be developed further to monitor the dependence of the neuronal action potential on nerve globins.
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