Title
Boosting tandem affinity purification of plant protein complexes Boosting tandem affinity purification of plant protein complexes
Author
Faculty/Department
Faculty of Sciences. Chemistry
Publication type
article
Publication
London ,
Subject
Biology
Source (journal)
Trends in plant science. - London
Volume/pages
13(2008) :10 , p. 517-520
ISSN
1360-1385
ISI
000260645400002
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Protein-interaction mapping based on the tandem affinity purification (TAP) approach has been successfully established for several systems, such as yeast and mammalian cells. However, relatively few protein complex purifications have been reported for plants. Here, we highlight solutions for the pitfalls and propose a major breakthrough in the quest for a better TAP tag in plants.
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