Publication
Title
Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of **Legionella pneumophila** and other **Legionella** species
Author
Abstract
A multiplex real-time PCR assay for detection of Legionella pneumophila and Legionella spp. and including an internal control was designed. Legionella species, L. pneumophila, and the internal control were detected simultaneously by probes labeled with 6-carboxy-fluorescein, hexachlorofluorescein, and indodicarbocyanine, respectively. Therefore, no postamplification analysis was required in order to distinguish the targets. The sensitivity of both assays was 2.5 CFU/ml, and from analysis of 10 culture-positive and 74 culture-negative samples from patients investigated for legionellosis, 100% agreement was observed by both assays in comparison to culture. Four additional positives were found by the multiplex real-time PCR assay in the Legionella culture-negative samples.
Language
English
Source (journal)
Journal of clinical microbiology. - Washington, D.C.
Publication
Washington, D.C. : 2003
ISSN
0095-1137
Volume/pages
41:9(2003), p. 4016-4021
ISI
000185246800002
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 14.01.2009
Last edited 12.11.2017
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