Title
A quantitative portrait of three xylanase inhibiting protein families in different wheat cultivars using 2D-DIGE and multivariate statistical tools A quantitative portrait of three xylanase inhibiting protein families in different wheat cultivars using 2D-DIGE and multivariate statistical tools
Author
Faculty/Department
Faculty of Sciences. Chemistry
Faculty of Sciences. Mathematics and Computer Science
Publication type
article
Publication
Subject
Chemistry
Biology
Human medicine
Source (journal)
Journal of proteomics
Volume/pages
72(2009) :3 , p. 484-500
ISSN
1874-3919
ISI
000265804800014
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Wheat grains contain three classes of xylanase inhibitors (XIs), i.e. TAXI (Triticum aestivum xylanase inhibitor), XIP (xylanase inhibiting protein) and TLXI (thaumatin-like xylanase inhibitor). These proteins are involved in plant defence and strongly affect cereal based processes in which inhibitor-sensitive xylanases are used. This paper reports on the successful use of 2D-DIGE and tandem MS to discriminate XI (iso)forms and measure their qualitative and quantitative variation in six different wheat cultivars. In total, 18 TAXI-, 27 XIP- and 3 TLXI-type XI spots were identified. The multiple members of the large TAXI-gene family make a considerable contribution to the total TAXI population. For XIP-type XIs, XIP-I is expressed as the predominant form, albeit under variable degrees of PTMs. Only one TLXI genetic variant was identified, showing different degrees of glycosylation. Multiple comparison analysis revealed up to 5-fold intercultivar differences in protein level of XI (iso)forms. Evaluation of abundance patterns using multivariate statistical tools revealed highly distinctive as well as correlated levels of different XI forms among the six cultivars. As the constitutive (and induced) levels of the different XI (iso)forms, which are differentially regulated in response to various forms of stress in other wheat plant parts, considerably vary between the cultivars, it can be assumed that their degree of resistance against pathogenic attack differs. Similarities in abundance profiles between XI (iso)forms and pathogenesis-related chitinases are also in line with a role in plant defence.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/76ba26/0eb0794d.pdf
E-info
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000265804800014&DestLinkType=RelatedRecords&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000265804800014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000265804800014&DestLinkType=CitingArticles&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
Handle