Publication
Title
Quality control for normal liquid based cytology: rescreening, high risk HPV targeted reviewing and/or high risk HPV detection?
Author
Abstract
The objective of this prospective study was to compare the number of CIN2+cases detected in negative cytology by different quality control (QC) methods. Full rescreening, high risk (HR) HPV targeted reviewing and HR HPV detection were compared. Randomly selected negative cytology detected by BD FocalPoint (NFR), by guided screening of the prescreened which needed further review (GS) and by manual screening (MS) was used. A 3 year follow-up period was available. Full rescreening of cytology only detected 23.5% of CIN2+cases, whereas the cytological rescreening of oncogenic positive slides (high risk HPV targeted reviewing) detected 7 of 17 CIN2+ cases (41.2%). Quantitative real-time PCR for 15 oncogenic HPV types detected all CIN2+ cases. Relative sensitivity to detect histological CIN2+ was 0.24 for full rescreening, 0.41 for HR targeted reviewing and 1.00 for HR HPV detection. In more than half of the reviewed negative cytological preparations associated with histological CIN2+cases no morphologically abnormal cells were detected despite a positive HPV test. The visual cut-off for the detection of abnormal cytology was established at 6.5 HR HPV copies/cell. High risk HPV detection has a higher yield for detection of CIN2+ cases as compared to manual screening followed by 5% full review, or compared to targeted reviewing of smears positive for oncogenic HPV types, and show diagnostic properties that support its use as a quality control procedure in cytologic laboratories.
Language
English
Source (journal)
Journal of cellular and molecular medicine
Publication
2009
ISSN
1582-1838
1582-4934
DOI
10.1111/J.1582-4934.2008.00379.X
Volume/pages
13 :9B (2009) , p. 4051-4060
ISI
000274179300095
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 03.04.2009
Last edited 06.12.2021
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