Title
The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteomeThe proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteome
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Research group
Proteinchemistry, proteomics and epigenetic signalling(PPES)
Publication type
article
Publication
Amsterdam,
Subject
Chemistry
Biology
Human medicine
Source (journal)
Biochimica et biophysica acta : proteins and proteomics. - Amsterdam
Volume/pages
1784(2008):7/8, p. 983-985
ISSN
1570-9639
ISI
000257563300002
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The human neuroblastoma cell line SH-SY5Y (ATCC: CRL-2266) is widely used as a neural cellular model system. The hitherto existing proteome data (115 proteins) are here extended. A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE with nano-LCMS/MS, N-terminal COFRADIC analysis with nano-LCMS/MS and 2D-PAGE with MALDI-TOF/TOF-MS peptide mass fingerprinting. The obtained proteome profile of this cell line is discussed.
E-info
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