Title
The basophil activation test in immediate drug allergy The basophil activation test in immediate drug allergy
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Leuven ,
Subject
Human medicine
Source (journal)
Acta clinica Belgica. - Leuven, 1946 - 1997
Volume/pages
64(2009) :2 , p. 129-135
ISSN
0001-5512
ISI
000265159600006
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Diagnosis of drug allergy is not always straight forward for several reasons. First, a broad spectrum of drugs can elicit various immune-mediated diseases with distinct pathomechanism, secondly, although exact epitope identification is not mandatory for clinical diagnosis, the epitope that causes the reaction is frequently unknown, thirdly in vitro or invivo test results might not be predictive of a clinical situation, and fi nally the gold standard or reference test for diagnosis, the drug challenge, is a complicated and sometimes dangerous endeavour. Upon challenge with specific allergens that cross-link membrane-bound IgE antibodies, basophils upregulate the expression of different activation markers such as CD63 and CD203c. These immunophenotypic alterations can be detected on a single-cell basis by multicolour fl ow cytometry using specifi c monoclonal antibodies in the basophil activation test (BAT). This review intends to summarise our current experience with the BAT in the diagnostic management of immediate-type allergy to drugs and related compounds that are generally (but not always) mediated by drug-specifi c IgE antibodies.
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