Publication
Title
The basophil activation test in immediate drug allergy
Author
Abstract
Diagnosis of drug allergy is not always straight forward for several reasons. First, a broad spectrum of drugs can elicit various immune-mediated diseases with distinct pathomechanism, secondly, although exact epitope identification is not mandatory for clinical diagnosis, the epitope that causes the reaction is frequently unknown, thirdly in vitro or invivo test results might not be predictive of a clinical situation, and fi nally the gold standard or reference test for diagnosis, the drug challenge, is a complicated and sometimes dangerous endeavour. Upon challenge with specific allergens that cross-link membrane-bound IgE antibodies, basophils upregulate the expression of different activation markers such as CD63 and CD203c. These immunophenotypic alterations can be detected on a single-cell basis by multicolour fl ow cytometry using specifi c monoclonal antibodies in the basophil activation test (BAT). This review intends to summarise our current experience with the BAT in the diagnostic management of immediate-type allergy to drugs and related compounds that are generally (but not always) mediated by drug-specifi c IgE antibodies.
Language
English
Source (journal)
Acta clinica Belgica. - Leuven, 1946 - 1997
Publication
Leuven : 2009
ISSN
0001-5512
Volume/pages
64:2(2009), p. 129-135
ISI
000265159600006
Full text (Publishers DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 16.04.2009
Last edited 31.03.2017
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