Publication
Title
Crystal structures of **T. vivax** nucleoside hydrolase in complex with new potent and specific inhibitors
Author
Abstract
Diseases caused by parasitic protozoa remain a major health problem, mainly due to old toxic drugs and rising drug resistance. Nucleoside hydrolases are key enzymes of the purine salvage pathway of parasites from the Trypanosomatidae family and are considered as possible drug targets. N-Arylmethyl substituted iminoribitols have been developed as selective nanomolar affinity inhibitors against the purine-specific nucleoside hydrolase of Trypanosoma vivax. The current paper describes the crystal structures of the T. vivax nucleoside hydrolase in complex with two of these inhibitors, to 1.3 and 1.85 Å resolution. These high resolution structures provide an accurate picture of the mode of binding of these inhibitors and their mechanism of transition-state mimicry, and are valuable tools to guide further inhibitor design. Comparison of the current structures with previously solved structures of the enzyme in complex with ground-state and transition-state-analogue inhibitors also allows for the elucidation of a detailed molecular mechanism of active-site loop opening/closing. These loop movements can be coupled to the complex kinetic mechanism of the T. vivax nucleoside hydrolase.
Language
English
Source (journal)
Biochimica et biophysica acta : proteins and proteomics. - Amsterdam, 2002, currens
Publication
Amsterdam : Elsevier , 2009
ISSN
1570-9639 [print]
1878-1454 [online]
DOI
10.1016/J.BBAPAP.2009.02.011
Volume/pages
(2009) , p. 953-960
ISI
000266387800011
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 20.04.2009
Last edited 25.05.2022
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