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Title
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Application of PCR-RFLP for the exploration of the molecular diversity of **Leishmania infantum** in Algeria
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Author
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Abstract
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| In recent years, new methods have been developed for the molecular typing of Leishmania that need to be extensively validated by studies of clinical isolates in a well defined epidemiological context. The present study is a contribution to this effort. Using PCR-RFLP of gp63 and cpb genes, we analysed 59 isolates of L. (L.) infantum obtained from different regions of Algeria and originating from different clinical forms, hosts and zymodemes. PCR-RFLP identified 15 different genotypes among the four zymodemes analysed, thereby demonstrating a higher discriminatory power than multilocus enzyme electrophoresis. We did not see any significant relationships between PCR-RFLP patterns and host origin. However, cpb polymorphism showed two interesting trends: a possible relationship with the cutaneous origin of the isolates and an association with a West-East cline. We verified the proof of evidence of the direct applicability of gp63 and cpb PCR-RFLP in blood samples from dogs. Further work is needed to compare the sensitivity of pattern detection with cpb and gp63 PCR-RFLP but our results pave the way to future multilocus PCR-RFLP studies of L. (L.) infantum populations. |
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Language
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English
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Source (journal)
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Transactions of the Royal Society of Tropical Medicine and Hygiene. - London
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Publication
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London
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2008
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ISSN
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0035-9203
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DOI
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10.1016/J.TRSTMH.2008.03.004
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Volume/pages
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102
:6
(2008)
, p. 556-563
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ISI
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000257518100009
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Full text (Publisher's DOI)
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