Title
|
|
|
|
First molecular epidemiological study of tuberculosis in Benin
| |
Author
|
|
|
|
| |
Abstract
|
|
|
|
OBJECTIVES: To assess the diversity of Mycobacterium tuberculosis strains in Cotonou, Benin, and the risk factors associated with clustering. METHODS: We analysed one sputum sample from 194 consecutive new pulmonary tuberculosis (TB) cases using two genotyping methods: spoligotyping and the 12 loci mycobacterial interspersed repetitive unit-variable number of tandem repeats (MIRU-VNTR). The data obtained were compared to the SpolDB4.0 database. RESULTS: We have found that spoligotype 61, highly predominant in West Africa, was also the most prevalent strain in Cotonou. We observed that the Beijing family represented 10.3% of strains and was associated with resistance to streptomycin. We also confirmed that combining spoligotyping and MIRU-VNTR provided a higher discriminatory power than the two techniques used individually. CONCLUSION: Spoligotype 61 and Beijing genotype are the most prevalent genotypes of M. tuberculosis in Cotonou. |
| |
Language
|
|
|
|
English
| |
Source (journal)
|
|
|
|
International journal of tuberculosis and lung disease. - Paris
| |
Publication
|
|
|
|
Paris
:
2009
| |
ISSN
|
|
|
|
1027-3719
| |
Volume/pages
|
|
|
|
13
:3
(2009)
, p. 317-322
| |
ISI
|
|
|
|
000263846700007
| |
|