Title
Performance of direct fluorescent antibody tests for routine diagnosis of **Chlamydia trachomatis** in Russian sexually transmitted disease clinics Performance of direct fluorescent antibody tests for routine diagnosis of **Chlamydia trachomatis** in Russian sexually transmitted disease clinics
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
London ,
Subject
Human medicine
Source (journal)
International journal of STD and AIDS / Association for Genito-Urinary Medicine [Belfast]; International Union Against the Venereal Diseases and the Treponematoses. - London
Volume/pages
19(2008) :12 , p. 851-855
ISSN
0956-4624
ISI
000261964900013
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Testing for Chlamydia trachomatis in Russia is usually done by microscopic examination of genital smears stained with fluorescent antibody provided in locally produced kits. The aim was to assess the sensitivity and specificity of such direct fluorescent antibody (DFA) tests compared with a nucleic acid amplification test (NAAT) (ligase chain reaction) to detect C. trachomatis in 171 cervical smears and 201 urethral smears from men. The patients were at high risk of chlamydial infection and had been recruited at three sexually transmitted disease clinics in Moscow. Among women, DFA test sensitivity was 6% (95% CI 014) and the specificity was 92% (95% CI 8897). Among men, the sensitivity was 9% (95% CI 216) and the specificity was 90% (95% CI 8394). Poor DFA test performance was probably due to poor antibody quality and such tests are not adequate for routine examination of populations with either low or high chlamydial prevalence. As there may remain a place for DFA testing where few patients are seen, the Russian Ministry of Health should enforce registration of diagnostic tests, and Russian manufacturers should seek ways of improving DFA test performance. However, the mainstay of testing should depend on NAATs.
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