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Title
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Cadmium and zinc-mediated oxidative burst in tobacco BY-2 cell suspension cultures
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Author
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Abstract
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| Generation of reactive oxygen species (ROS) constitutes an important first reaction under many stress conditions in plants. We demonstrate that Nicotiana tabacum L. cv. Bright Yellow 2 (TBY-2) cells in suspension cultures, generate superoxide radical and hydrogen peroxide upon treatment with cadmium and zinc. Addition of catalase and N,N-diethyldithiocarbamate (DDC) decreased the level of H2O2, whereas superoxide dismutase (SOD) induced a slight increase of the H2O2 production. The effects of catalase, DDC and SOD on the heavy metal-induced ROS production indicate that it occurs outside of the cells, and that at least part of the hydrogen peroxide is produced by dismutation of the superoxide radical (O2 ·−). The effect of pretreatment of the cell cultures with commonly used mammalian NADPH oxidase inhibitors was also tested. Strong inhibitions of cadmium and zinc-mediated ROS production were obtained with the flavoprotein inhibitorsdiphenylene iodonium (DPI) and quinacrine and with an inhibitor of b-type cytochromesimidazol. Membrane permeable-N-ethyl maleimide (NEM) and iodoacetate, and membrane non-permeable thiol reagentspara-chloromercuribenzoic acid (pCMBS) also inhibited the ROS production. These results suggested that the enzyme responsible for cadmium and zinc-induced ROS production in tobacco cells contains a flavocytochrome. They also show the importance of intra- and extracellular thiol groups in the observed stress reaction. The induction of ROS production with heavy metals showed properties comparable to the elicitor-induced oxidative burst in other plant cells. |
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Language
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English
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Source (journal)
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Acta physiologiae plantarum
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Publication
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2009
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ISSN
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0137-5881
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DOI
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10.1007/S11738-008-0197-8
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Volume/pages
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31
:1
(2009)
, p. 43-49
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ISI
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000261936000005
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Full text (Publisher's DOI)
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