Title
Cadmium and zinc-mediated oxidative burst in tobacco BY-2 cell suspension cultures Cadmium and zinc-mediated oxidative burst in tobacco BY-2 cell suspension cultures
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Subject
Biology
Source (journal)
Acta physiologiae plantarum
Volume/pages
31(2009) :1 , p. 43-49
ISSN
0137-5881
ISI
000261936000005
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Generation of reactive oxygen species (ROS) constitutes an important first reaction under many stress conditions in plants. We demonstrate that Nicotiana tabacum L. cv. Bright Yellow 2 (TBY-2) cells in suspension cultures, generate superoxide radical and hydrogen peroxide upon treatment with cadmium and zinc. Addition of catalase and N,N-diethyldithiocarbamate (DDC) decreased the level of H2O2, whereas superoxide dismutase (SOD) induced a slight increase of the H2O2 production. The effects of catalase, DDC and SOD on the heavy metal-induced ROS production indicate that it occurs outside of the cells, and that at least part of the hydrogen peroxide is produced by dismutation of the superoxide radical (O2 ·−). The effect of pretreatment of the cell cultures with commonly used mammalian NADPH oxidase inhibitors was also tested. Strong inhibitions of cadmium and zinc-mediated ROS production were obtained with the flavoprotein inhibitorsdiphenylene iodonium (DPI) and quinacrine and with an inhibitor of b-type cytochromesimidazol. Membrane permeable-N-ethyl maleimide (NEM) and iodoacetate, and membrane non-permeable thiol reagentspara-chloromercuribenzoic acid (pCMBS) also inhibited the ROS production. These results suggested that the enzyme responsible for cadmium and zinc-induced ROS production in tobacco cells contains a flavocytochrome. They also show the importance of intra- and extracellular thiol groups in the observed stress reaction. The induction of ROS production with heavy metals showed properties comparable to the elicitor-induced oxidative burst in other plant cells.
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