Title
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Cryotolerance of bovine blastocysts is affected by oocyte maturation in media containing palmitic or stearic acid
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Author
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Abstract
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In this study, non-esterified fatty acids (NEFAs) were added during in vitro maturation at concentrations measured previously in follicular fluid (FF) of high-producing dairy cows in a negative energy status to evaluate their subsequent effect on the embryos cryotolerance. Oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of NEFAs dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in synthetic oviduct fluid medium supplemented with 5% FCS. Embryos that had at least reached the blastocyst stage were vitrified by open pulled straw (OPS) vitrification. Addition of palmitic (C16 : 0) or stearic acid (C18 : 0) during oocyte maturation had significant negative effects on embryo cryotolerance, whereas ethanol or oleic acid (C18 : 1) had no effect. These in vitro results suggest that high NEFA concentrations in FF during a period of negative energy balance in high-yielding dairy cows can have carry-over effects on embryo quality. |
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Language
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English
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Source (journal)
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Reproduction in domestic animals. - Berlin
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Publication
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Berlin
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2009
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ISSN
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0936-6768
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DOI
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10.1111/J.1439-0531.2008.01084.X
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Volume/pages
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44
:1
(2009)
, p. 140-142
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ISI
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000262487500024
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Full text (Publisher's DOI)
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