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Title
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CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset
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Author
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Abstract
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| The mitosis-to-endocycle transition requires the controlled inactivation of M-phase-associated cyclin-dependent kinase (CDK) activity. Previously, the B-type CDKB1;1 has been identified as an important negative regulator of endocycle onset. Here, we demonstrate that CDKB1;1 co-purifies and associates with the A2-type cyclin CYCA2;3. Co-expression of CYCA2;3 with CDKB1;1 triggered ectopic cell divisions and inhibited endoreduplication. Moreover, the enhanced endoreduplication phenotype observed after overexpression of a dominant-negative allele of CDKB1;1 could be partially complemented by CYCA2;3 co-overexpression, illustrating that both subunits unite in vivo to form a functional complex. CYCA2;3 protein stability was found to be controlled by CCS52A1, an activator of the anaphase-promoting complex. We conclude that CCS52A1 participates in the endocycle onset by down-regulating CDKB1;1 activity through the destruction of CYCA2;3. |
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Language
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English
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Source (journal)
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Plant physiology. - Rockville, Md
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Publication
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Rockville, Md
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2009
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ISSN
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0032-0889
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DOI
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10.1104/PP.109.140269
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Volume/pages
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150
:3
(2009)
, p. 1482-1493
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ISI
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000268696800031
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Full text (Publisher's DOI)
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