Title
CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset
Author
Faculty/Department
Faculty of Sciences. Chemistry
Publication type
article
Publication
Rockville, Md ,
Subject
Biology
Source (journal)
Plant physiology. - Rockville, Md
Volume/pages
150(2009) :3 , p. 1482-1493
ISSN
0032-0889
ISI
000268696800031
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The mitosis-to-endocycle transition requires the controlled inactivation of M-phase-associated cyclin-dependent kinase (CDK) activity. Previously, the B-type CDKB1;1 has been identified as an important negative regulator of endocycle onset. Here, we demonstrate that CDKB1;1 co-purifies and associates with the A2-type cyclin CYCA2;3. Co-expression of CYCA2;3 with CDKB1;1 triggered ectopic cell divisions and inhibited endoreduplication. Moreover, the enhanced endoreduplication phenotype observed after overexpression of a dominant-negative allele of CDKB1;1 could be partially complemented by CYCA2;3 co-overexpression, illustrating that both subunits unite in vivo to form a functional complex. CYCA2;3 protein stability was found to be controlled by CCS52A1, an activator of the anaphase-promoting complex. We conclude that CCS52A1 participates in the endocycle onset by down-regulating CDKB1;1 activity through the destruction of CYCA2;3.
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