Title
The effect of okadaic acid on **Arabidopsis thaliana** root morphology and microtubule organization in its cells The effect of okadaic acid on **Arabidopsis thaliana** root morphology and microtubule organization in its cells
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
New York ,
Subject
Biology
Source (journal)
Cytology and genetics / Polska Akademia Nauk. Komitet Nauk Chemicznych. - New York
Volume/pages
43(2009) :1 , p. 1-8
ISSN
0095-4527
ISI
000265507300001
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
To investigate the role of protein hyperphosphorylation in plant cells, the effect of okadaic acid, a specific inhibitor of protein phosphatases PPI and PP2A, on the general morphology of Arabidopsis thaliana primary roots and the structural-functional characteristics of cortical microtubules in different cell types in all primary root growth zones was studied. It was found that okadaic acid affects microtubule organization in a different manner depending on the type of cells and functional zones of the primary root. Cortical microtubules in the epidermis and cortex cells of the elongation zone proved to be most sensitive to 0.1, 1, and 10 nM okadaic acid which completely depolymerized after inhibitor treatment. In trichoblasts, atrichoblasts of differentiation zone treatment with okadaic acid caused the microtubules stabilization. The treatment with okadaic acid significantly affected the morphology of root hairs, causing their swelling and branching as a result of abnormal microtubule orientation. The results of this study suggest that induction of protein hyperphosphorylation as a result of protein phosphatase inhibition plays a crucial key in microtubule organization in plant cells.
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