Title
Feasibility of collecting self-sampled vaginal swabs by mail: quantity and quality of genomic DNA Feasibility of collecting self-sampled vaginal swabs by mail: quantity and quality of genomic DNA
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Wiesbaden ,
Subject
Human medicine
Source (journal)
European journal of clinical microbiology and infectious diseases. - Wiesbaden
Volume/pages
28(2009) :11 , p. 1285-1289
ISSN
0934-9723
ISI
000271404400001
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Vaginal self-sampling may be valuable as an alternative method of cervical cancer screening in areas of poor resources, to enrol women who, otherwise, would not participate in population-based cervical cancer screening and in epidemiological follow-up studies. We assessed the reliability of mailed vaginal samples by evaluating the quantity and quality of genomic DNA in the samples. Mailed swabs (n = 201) were compared with freshly collected samples (n = 200) for DNA concentration (45.1 versus 50.9 ng/µl, respectively) and purity (mean optical density [OD] 260/280 ratio 1.88 versus 1.78, respectively). A small, non-significant, decrease in DNA yield with longer transport time was noted. The DNA yield of mailed samples was significantly lower compared to fresh samples (P < 0.002), but this lower yield had little effect on polymerase chain reaction (PCR) amplification. In conclusion, the large majority of mailed self-sampled vaginal swabs resulted in DNA of adequate purity and concentration for further research.
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