Publication
Title
Feasibility of collecting self-sampled vaginal swabs by mail: quantity and quality of genomic DNA
Author
Abstract
Vaginal self-sampling may be valuable as an alternative method of cervical cancer screening in areas of poor resources, to enrol women who, otherwise, would not participate in population-based cervical cancer screening and in epidemiological follow-up studies. We assessed the reliability of mailed vaginal samples by evaluating the quantity and quality of genomic DNA in the samples. Mailed swabs (n = 201) were compared with freshly collected samples (n = 200) for DNA concentration (45.1 versus 50.9 ng/µl, respectively) and purity (mean optical density [OD] 260/280 ratio 1.88 versus 1.78, respectively). A small, non-significant, decrease in DNA yield with longer transport time was noted. The DNA yield of mailed samples was significantly lower compared to fresh samples (P < 0.002), but this lower yield had little effect on polymerase chain reaction (PCR) amplification. In conclusion, the large majority of mailed self-sampled vaginal swabs resulted in DNA of adequate purity and concentration for further research.
Language
English
Source (journal)
European journal of clinical microbiology and infectious diseases. - Wiesbaden
Publication
Wiesbaden : 2009
ISSN
0934-9723
Volume/pages
28:11(2009), p. 1285-1289
ISI
000271404400001
Full text (Publishers DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 27.07.2009
Last edited 19.05.2017
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