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Title
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Feasibility of collecting self-sampled vaginal swabs by mail: quantity and quality of genomic DNA
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Author
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Abstract
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| Vaginal self-sampling may be valuable as an alternative method of cervical cancer screening in areas of poor resources, to enrol women who, otherwise, would not participate in population-based cervical cancer screening and in epidemiological follow-up studies. We assessed the reliability of mailed vaginal samples by evaluating the quantity and quality of genomic DNA in the samples. Mailed swabs (n = 201) were compared with freshly collected samples (n = 200) for DNA concentration (45.1 versus 50.9 ng/µl, respectively) and purity (mean optical density [OD] 260/280 ratio 1.88 versus 1.78, respectively). A small, non-significant, decrease in DNA yield with longer transport time was noted. The DNA yield of mailed samples was significantly lower compared to fresh samples (P < 0.002), but this lower yield had little effect on polymerase chain reaction (PCR) amplification. In conclusion, the large majority of mailed self-sampled vaginal swabs resulted in DNA of adequate purity and concentration for further research. |
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Language
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English
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Source (journal)
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European journal of clinical microbiology and infectious diseases. - Wiesbaden
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Publication
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Wiesbaden
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2009
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ISSN
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0934-9723
[print]
1435-4373
[online]
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DOI
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10.1007/S10096-009-0776-7
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Volume/pages
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28
:11
(2009)
, p. 1285-1289
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ISI
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000271404400001
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Full text (Publisher's DOI)
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