Publication
Title
Human C-reactive protein activates monocyte-derived dendritic cells and induces dendritic cell-mediated T-cell activation
Author
Abstract
Objective-: Recent studies proposed a pathogenic role for C-reactive protein (CRP), an independent predictor of cardiovascular disease (CVD), in atherosclerosis. Therefore, we tested whether CRP may modulate dendritic cell (DC) function, because these professional antigen-presenting cells have been implicated in atherogenesis. Methods and Results-: Human monocyte-derived immature DCs were cultured with human CRP (0 to 60 [mu]g/mL) for 24 hours. Thereafter, activation markers were measured by flow-cytometry and DCs were cocultured with CFSE-labeled lymphocytes to measure T-cell proliferation and interferon (IFN)-[gamma] secretion after 8 days. Exposure to 60 [mu]g/mL CRP (n=5) induced an activated cell morphology and significant (CD40 increase MFI 5.23+/-0.28, P<0.01 paired t test; CD80 6.18+/-0.51, P<0.01) to modest (CD83 1.38+/-0.17, P<0.05, CCR7 1.60+/-0.29, P=0.05) upregulation of DC activation markers. The expression of CD86 and HLA-DR was high, but not affected. T-lymphocytes incubated with CRP-pulsed DCs displayed increased IFN-[gamma] secretion and proliferation (P<0.001). DC activation was concentration-dependent and detected from 2 [mu]g/mL CRP; the maximum effect was equivalent to that seen with 0.1 [mu]g/mL lipopolysaccharide (LPS). Polymyxin B abolished the LPS response, without influencing CRP effects. Finally, immunohistochemistry could demonstrate DC/CRP colocalization in human atherosclerotic lesions. Conclusions-: These findings suggest that CRP in plaques or found circulating in CVD patients can influence DC function during atherogenesis.
Language
English
Source (journal)
Arteriosclerosis, thrombosis, and vascular biology / American Heart Association. - Dallas, Tex., 1995, currens
Publication
Dallas, Tex. : 2008
ISSN
1079-5642
1524-4636 [online]
Volume/pages
28:3(2008), p. 511-518
ISI
000253429000026
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 13.10.2009
Last edited 03.08.2017
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