Title
Quantitative DNA-methylation in **Daphnia magna** and effects of multigeneration Zn exposure Quantitative DNA-methylation in **Daphnia magna** and effects of multigeneration Zn exposure
Author
Faculty/Department
Faculty of Sciences. Chemistry
Publication type
article
Publication
Oxford ,
Subject
Chemistry
Biology
Source (journal)
Comparative biochemistry and physiology : C : toxicology & pharmacology. - Oxford, 2000, currens
Volume/pages
150(2009) :3 , p. 343-348
ISSN
1532-0456
ISI
000269231400003
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Little research on the epigenetic phenomenon of DNA methylation has been performed with invertebrates. However, a few studies have shown that effects of transient chemical exposure can be transferred to non-exposed generations through epigenetic inheritance. If this is a wide-spread phenomenon it may have major consequences for current ecological risk assessments. The presence of DNA methylation in Daphnia magna has only very recently been demonstrated, although not quantified. In the present study, the overall content of 5-methyl-2-deoxycytidine (5mdC) in the DNA of D. magna was determined. Additionally, the effect of transgenerational Zn exposure on the 5mdC content was assessed. An optimized LC-MS-MS method proved to be very suitable for measuring overall levels of 5mdC in relatively small samples of D. magna DNA. The average [5mdC]/[dG] ratio ranged from 0.13% to 0.81%. A slight but significant decrease in DNA methylation was found in the offspring (F1) of Zn exposed daphnids in the parental generation. However, this effect did not propagate into the next generation (F2). The presence of DNA methylation in D. magna and the finding that different exposure histories can entail different levels of methylation, open the way for further investigations on the implications of epigenetics in aquatic toxicology.
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