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Title
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Sensitive and selective method for the determination of bisphenol-A and triclosan in serum and urine as pentafluorobenzoate-derivatives using GCECNI/MS
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Author
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Abstract
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| The development and validation of an analytical method is presented for the determination of bisphenol-A (BPA) and triclosan (TCS), two ubiquitous contaminants, in serum and urine. The glucuronidated metabolites were first turned into their free forms to determine total BPA and TCS. The determination consisted of a solid-phase extraction on Oasis HLB cartridges followed by an extractive derivatization with pentafluorobenzoylchloride. The extract was then purified on 10% (w/w) acidified silica and analyzed by gas chromatographymass spectrometry in electron-capture negative ionization mode. Monitored ions were m/z 616 and 406 for BPA and m/z 482 and 287 for TCS, respectively. Limits of quantification were 0.5 ng/mL in serum and 0.2 ng/mL in urine for BPA and 0.1 ng/mL in serum and 0.05 ng/mL in urine for TCS. Method recoveries were between 76 and 110%, while repeatability was below 20%. The method was applied on 20 serum and 20 urine samples. The detection frequency in serum was 10% and 55% for BPA and TCS, respectively. BPA and TCS could be detected in all urine samples with median concentrations of 1.25 ng BPA/mL (range 0.585.20 ng/mL) and 1.71 ng TCS/mL (0.18672 ng/mL). |
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Language
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English
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Source (journal)
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Journal of chromatography : B: analytical technologies in the biomedical and life sciences. - Amsterdam, 2002, currens
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Publication
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Amsterdam
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Elsevier
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2009
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ISSN
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1570-0232
[print]
1873-376X
[online]
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DOI
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10.1016/J.JCHROMB.2009.10.017
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Volume/pages
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877
:31
(2009)
, p. 4042-4046
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ISI
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000272317600016
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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