Title
In vivo antioxidative activity of a quantified **Pueraria lobata** root extract In vivo antioxidative activity of a quantified **Pueraria lobata** root extract
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Lausanne ,
Subject
Pharmacology. Therapy
Source (journal)
Journal of ethnopharmacology. - Lausanne
Volume/pages
127(2010) :1 , p. 112-117
ISSN
0378-8741
ISI
000274715400018
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Aim of the study Oxidative stress has been associated with many pathological disorders such as atherosclerosis, diabetes and cancer. Supplementation with exogenous antioxidants, including phenolic compounds from plant sources, may help to restore the pro-oxidative/antioxidative balance. To take into account effects of absorption, metabolisation, plasma protein binding, distribution, and elimination, antioxidative research should not be limited to in vitro assays but be extended to in vivo models. Materials and methods In the present work a quantified 50% EtOH root extract of Pueraria lobata (Willd.) Ohwi (Fabaceae) was selected to determine its in vivo antioxidative activity in a diabetic rat model, where diabetes and the accompanying oxidative stress were induced by intraperitoneal administration of streptozotocin. This root extract was found to contain 10.42 } 0.15% puerarin as the main constituent and smaller amounts of the related isoflavonoids 3-hydroxypuerarin, 3-methoxypuerarin, 6-xylosylpuerarin, daidzin, genistin, daidzein and genistein, as determined by a validated HPLC method. This extract was administered orally at a daily dose of 500 mg/kg root extract, corresponding to 50 mg/kg puerarin, during 3 weeks. In addition the effect on the plasma concentration of some fat-soluble antioxidants (co-enzyme Q9, ¿- and Á-tocopherol) was evaluated. Results and conclusions The level of malondialdehyde (MDA) in plasma, used as a marker of oxidative damage to lipids, was reduced to the same level as in healthy control animals, and as in the positive control group treated daily with 50 mg/kg ¿-tocopherol acetate. No obvious signs of toxicity were observed by administration of 10~ the treatment dose. Graphical abstract In a diabetic rat model, where diabetes was induced by streptozotocin, oral administration of a quantified Pueraria lobata root extract at a daily dose of 500 mg/kg, corresponding to 50 mg/kg puerarin, during 3 weeks, resulted in a reduction of the level of malondialdehyde (MDA) in plasma, used as a marker of oxidative damage to lipids, to the same level as in healthy control animals, and to the same level as in the positive control group treated with 50 mg/kg ¿-tocopherol acetate.
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