Title
PSK-<tex>$\alpha$</tex> promotes root growth in Arabidopsis PSK-<tex>$\alpha$</tex> promotes root growth in Arabidopsis
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Oxford ,
Subject
Biology
Source (journal)
New phytologist. - Oxford
Volume/pages
181(2009) :4 , p. 820-831
ISSN
0028-646X
ISI
000263257100010
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Phytosulfokine-á (PSK-á) is a disulfated pentapeptide described to act as a growth factor in suspension cells. In this study, the involvement of PSK signaling through the PSK receptor gene AtPSKR1 in Arabidopsis root growth was assessed. Expression studies of PSK precursor genes and of AtPSKR1 were performed in roots with RT-PCR and P:GUS analyses. Root elongation, lateral root formation, cell production and root cell elongation were analyzed in wild-type (wt) and in the receptor knockout mutant Atpskr1-T treated with or without synthetic PSK-á. Phytosulfokine and AtPSKR1 genes are differentially expressed in roots. PSK-á induced root growth in a dose-dependent manner without affecting lateral root density. Kinematic analysis established that enhancement of root growth by PSK-á was mainly caused by an increase in cell size. In Atpskr1-T, the primary roots were shorter as a result of reduced mature cell size and a smaller root apical meristem composed of fewer cells than in wt. The results indicate that PSK-á signaling through AtPSKR1 affects root elongation primarily via control of mature cell size. Root organogenesis, on the other hand, is not controlled by PSK-á.
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