Title
Overexpression of the **Arabidopsis** anaphase promoting complex subunit **CDC27a** increases growth rate and organ sizeOverexpression of the **Arabidopsis** anaphase promoting complex subunit **CDC27a** increases growth rate and organ size
Author
Faculty/Department
Faculty of Sciences. Biology
Research group
Integrated Molecular Plant Physiology Research (IMPRES)
Molecular Plant Physiology and Biotechnology
Publication type
article
Publication
The Hague,
Subject
Biology
Source (journal)
Plant molecular biology: an international journal on molecular biology, biochemistry and genetic engineering. - The Hague
Volume/pages
71(2009):3, p. 307-318
ISSN
0167-4412
ISI
000269152200008
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The Anaphase Promoting Complex (APC) controls CDK activity by targeting the ubiquitin-dependent proteolysis of S-phase and mitosis-promoting cyclins. Here, we report that the ectopic expression of the Arabidopsis CDC27a, an APC subunit, accelerates plant growth and results in plants with increased biomass production. CDC27a overexpression was associated to apical meristem restructuration, protoplasts with higher 3H-thimidine incorporation and altered cell-cycle marker expression. Total protein extracts immunoprecipitated with a CDC27a antibody showed ubiquitin ligase activity, indicating that the Arabidopsis CDC27a gets incorporated into APC complexes. These results indicate a role of AtCDC27a in regulation of plant growth and raise the possibility that the activity of the APC and the rates of plant cell division could be regulated by the concentration of the CDC27a subunit.
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