Publication
Title
Overexpression of the **Arabidopsis** anaphase promoting complex subunit **CDC27a** increases growth rate and organ size
Author
Abstract
The Anaphase Promoting Complex (APC) controls CDK activity by targeting the ubiquitin-dependent proteolysis of S-phase and mitosis-promoting cyclins. Here, we report that the ectopic expression of the Arabidopsis CDC27a, an APC subunit, accelerates plant growth and results in plants with increased biomass production. CDC27a overexpression was associated to apical meristem restructuration, protoplasts with higher 3H-thimidine incorporation and altered cell-cycle marker expression. Total protein extracts immunoprecipitated with a CDC27a antibody showed ubiquitin ligase activity, indicating that the Arabidopsis CDC27a gets incorporated into APC complexes. These results indicate a role of AtCDC27a in regulation of plant growth and raise the possibility that the activity of the APC and the rates of plant cell division could be regulated by the concentration of the CDC27a subunit.
Language
English
Source (journal)
Plant molecular biology: an international journal on molecular biology, biochemistry and genetic engineering. - The Hague
Publication
The Hague : 2009
ISSN
0167-4412
Volume/pages
71:3(2009), p. 307-318
ISI
000269152200008
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 22.01.2010
Last edited 06.07.2017