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Title
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Phage display as a novel screening tool for primary toxicological targets
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Author
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Abstract
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| In the present study the use of phage display as a screening tool to determine primary toxicological targets was investigated. These primary toxicological targets are the targets in the cell with which a chemical compound initially interacts and that are responsible for consecutive (toxic) effects. Nickel was used as model compound for the present study. By selection of Ni-binding peptides out of a 12-mer peptide phage library, it was possible to identify primary toxicological targets of Ni (and other metals). The selected Ni-binding peptides showed similarities to important primary toxicological targets of Ni, such as the hydrogenase nickel incorporation protein (hypB) and the Mg/Ni/Co transporter (corA). This shows that phage display, which is already widely used in other research fields, also has potential in ecotoxicology, as a novel screening tool with which to determine primary toxicological targets of chemical compounds. |
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Language
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English
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Source (journal)
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Environmental toxicology and chemistry. - New York, N.Y., 1982, currens
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Publication
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New York, N.Y.
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2010
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ISSN
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0730-7268
[print]
1552-8618
[online]
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Volume/pages
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29
:2
(2010)
, p. 250-255
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ISI
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000274272500003
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Full text (Publisher's DOI)
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